Immunological Investigations of Cytochrome c

Immunological activity of cytochrome c. 3. Enhancement of antibody detection and immune response initiation by cytochrome c polymers.

Antibodies which fail to precipitate with the monomeric homologous cytochromes c were elicited in rabbits by the conjugate of bovine y-globulin and cytochrome c (tuna and turkey proteins) or by free monomeric cytochrome c (kangaroo and, in one case, the horse protein). Such antibodies do form precipitates and fix complement when allowed to react with the polymerized homologous antigen. In contr...

Immunological activity of cytochrome c. I. Precipitating antibodies to monomeric vertebrate cytochromes c.

The production of antibodies by rabbits in response to monomeric preparations of human, Macaca mulatta, and horse cytochromes c, incorporated in complete Freund’s adjuvant, is described. These antibodies precipitate with the monomeric homologous antigens, whereas no such precipitates occurred with antisera elicited by monomeric preparations of the kangaroo (Macropus canguru), turkey, and tuna p...

Immunological and Chemical Investigations of Vaccine Virus

Washed elementary bodies obtained from dermal vaccine virus contain ash, carbohydrate, fat, and nitrogen a part of which is undoubtedly in the form of protein. These components are similar to those found in bacteria and other substances of protoplasmic origin.

Laboratory Investigations and Immunological Testing in Sarcoidosis

Investigations of dioxygen reactivity with synthetic models of the metalloproteins hemocyanin and cytochrome c oxidase

Synthetic, chemical, and low-temperature stopped-flow kinetic studies in EtCN indicate initial formation of the 1:l 0 2 adduct [(TMPA)Cu(O2)]+ (4), and subsequent formation of the 2:1 0 2 adduct [( ( T M P A > C U ] ~ ( O ~ ) ] ~ + (2) from [ (TMPA)Cu(RCN)]+ (1) and 0 2 ; vacuum-cycling experiments demonstrate reversible binding of a. An X-ray structural study of 2 revealed a trans-p-1, 2 confi...